Name: CARLOS ANDRÉ CUNHA DA SILVA
Publication date: 09/03/2022
Advisor:
Name |
Role |
|---|---|
| MOISES PALACI | Advisor * |
Examining board:
| Name |
Role |
|---|---|
| LILIANA CRUZ SPANO | Internal Examiner * |
| MOISES PALACI | Advisor * |
| SOLANGE ALVES VINHAS | External Examiner * |
Summary: Tuberculosis (TB) is humanity`s oldest infectious disease, and it still represents a major challenge today. Caused by species of the Mycobacterium tuberculosis Complex (CMTB), the disease has a wide distribution, predominantly in the poorest populations and in countries with lower socioeconomic development. A major problem related to TB is latent infection, in which the bacterium is in the host in a quiescent form
denominated dormancy. Despite recent studies breaking the paradigm of the binary
model of interaction between MTB and the host, which consisted of the forms of
disease (active) and latent infection (LTBI), little is known about the physiological state
of MTB during the latency period. In this context, this work aims to study genetic
alterations in secondary cases from their respective index cases and to investigate
possible alterations and their biological implications. Bioinformatics analyzes were
used in 24 strains, through comparative genomics between index cases and their
respective secondary cases using RAST, Snippy and Spades software. The genetic
differences that led to some modification in the secondary case were called variants
or variations. Data analysis indicated that most of the variations found were in the
metabolic subsystems related to virulence and membrane transport, we also found
that in the subsystems related to dormancy and stress response there were no
variations. There are no genetic alterations that lead to loss or gain of M. tuberculosis
resistance during the dormancy period in secondary cases.
